Peptide Synthesis and Applications
John Howl
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
Կատեգորիաներ:
Տարի:
2005
Հրատարակում:
1
Հրատարակչություն:
Humana Press
Լեզու:
english
Էջեր:
273
ISBN 10:
1592598773
ISBN 13:
9781592598779
Սերիաներ:
Methods in Molecular Biology
Ֆայլ:
PDF, 7.01 MB
IPFS:
,
english, 2005